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10th March 2010
| | P | W | D | L | F | A | +/- | Pts |
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| 1 | Dartford | 28 | 20 | 4 | 4 | 69 | 28 | +41 | 64 |
| 2 | Kingstonian | 32 | 16 | 5 | 11 | 58 | 52 | +6 | 53 |
| 3 | Sutton United | 30 | 15 | 6 | 9 | 48 | 32 | +16 | 51 |
| 4 | Hastings United | 32 | 14 | 8 | 10 | 51 | 42 | +9 | 50 |
| 5 | Boreham Wood | 31 | 14 | 4 | 13 | 42 | 37 | +5 | 46 |
| 6 | Wealdstone | 28 | 12 | 10 | 6 | 48 | 46 | +2 | 46 |
| 7 | Horsham | 30 | 13 | 5 | 12 | 52 | 46 | +6 | 44 |
| 8 | Canvey Island | 30 | 12 | 8 | 10 | 45 | 42 | +3 | 44 |
| 9 | Tooting & Mitcham United | 32 | 12 | 8 | 12 | 44 | 46 | -2 | 44 |
| 10 | Harrow Borough | 31 | 11 | 10 | 10 | 48 | 45 | +3 | 43 |
| 11 | Aveley | 29 | 12 | 5 | 12 | 56 | 49 | +7 | 41 |
| 12 | AFC Hornchurch | 30 | 10 | 11 | 9 | 35 | 33 | +2 | 41 |
| 13 | Ashford Town (Mx) | 31 | 10 | 10 | 11 | 54 | 59 | -5 | 40 |
| 14 | Tonbridge Angels | 30 | 11 | 6 | 13 | 49 | 55 | -6 | 39 |
| 15 | Hendon | 29 | 11 | 5 | 13 | 42 | 42 | 0 | 38 |
| 16 | Cray Wanderers | 32 | 11 | 5 | 16 | 42 | 59 | -17 | 38 |
| 17 | Maidstone United | 30 | 10 | 7 | 13 | 28 | 37 | -9 | 37 |
| 18 | Billericay Town | 30 | 9 | 9 | 12 | 32 | 34 | -2 | 36 |
| 19 | Carshalton Athletic | 30 | 8 | 9 | 13 | 42 | 50 | -8 | 33 |
| 20 | Bognor Regis Town | 30 | 7 | 10 | 13 | 35 | 47 | -12 | 31 |
| 21 | Waltham Abbey | 29 | 8 | 6 | 15 | 35 | 51 | -16 | 30 |
| 22 | Margate | 30 | 7 | 7 | 16 | 36 | 59 | -23 | 28 |
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